<i>Tripterygium regelii</i> decreases the biosynthesis of triacylglycerol and cholesterol in HepG2 cells

<p>In the course of screening to find a plant material decreasing the activity of triacylglycerol and cholesterol, we identified <i>Tripterygium regelii</i> (TR). The methanol extract of TR leaves (TR-LM) was shown to reduce the intracellular lipid contents consisting of triacylglycerol (TG) and cholesterol in HepG2 cells. TR-LM also downregulated the mRNA and protein expression of the lipogenic genes such as SREBP-1 and its target enzymes. Consequently, TR-LM reduced the TG biosynthesis in HepG2 cells. In addition, TR-LM decreased SREBP2 and its target enzyme HMG-CoA reductase, which is involved in cholesterol synthesis. In this study, we evaluated that TR-LM attenuated cellular lipid contents through the suppression of <i>de novo</i> TG and cholesterol biosynthesis in HepG2 cells. All these taken together, TR-LM could be beneficial in regulating lipid metabolism and useful preventing the hyperlipidemia and its complications, in that liver is a crucial tissue for the secretion of serum lipids.</p> <p>The methanol extract of <i>Tripterygium regelii</i> leaves reduced intracellular contents of TG and cholesterol in HepG2 cells.</p>