(i) The automated microarray system. (ii) Structure of the automated microarray system. A, protein chip; B, chip and reagent holder; C, digital video camera; D, control and analysis PC; E, belt-drive system; F, pumps; G, needle holder; r1, serum; r2, enzyme-labeled antibody; r3, chemiluminescence reagent; r4, TBS; r5, TBST; r6, liquid waste. (iii) Movements of the automated microarray system. Procedure; Step 1 (preparation for reaction): At Position I, set a protein chip (A) to the holder (B). Inject serum (r1) onto the chip and stir. Inject enzyme-labeled antibody (r2) and chemilumigenic reagent (r3) to the reagent holder (B). Step 2 (wash): The holder moves to Position III, and serum is wasted out. TBS (r4) is injected and wasted, and then TBST (r5) is injected and wasted. Step 3 (reaction): The holder moves to Position II, and r2 is sucked up to the needle. Moves to Position III, r2 is injected on the chip, and stir. Step 4 (wash): The holder moves to Position III, and b is wasted o

(i) The automated microarray system. (ii) Structure of the automated microarray system. A, protein chip; B, chip and reagent holder; C, digital video camera; D, control and analysis PC; E, belt-drive system; F, pumps; G, needle holder; r1, serum; r2, enzyme-labeled antibody; r3, chemiluminescence reagent; r4, TBS; r5, TBST; r6, liquid waste. (iii) Movements of the automated microarray system. Procedure; Step 1 (preparation for reaction): At Position I, set a protein chip (A) to the holder (B). Inject serum (r1) onto the chip and stir. Inject enzyme-labeled antibody (r2) and chemilumigenic reagent (r3) to the reagent holder (B). Step 2 (wash): The holder moves to Position III, and serum is wasted out. TBS (r4) is injected and wasted, and then TBST (r5) is injected and wasted. Step 3 (reaction): The holder moves to Position II, and r2 is sucked up to the needle. Moves to Position III, r2 is injected on the chip, and stir. Step 4 (wash): The holder moves to Position III, and b is wasted out. TBS (r4) is injected and wasted, and then TBST (r5) is injected and wasted. Step 5 (preparation for imaging): The holder moves to Position II, and r3 is sucked up to the needle. Moves to Position III, and r3 is injected onto the chip. Step 6 (imaging): The holder moves to Position IV and images are taken by the digital video camera (C). Step 7 (completion): The holder moves back to Position I, and the chip is removed.