pntd.0005306.g003.tif (238.86 kB)
S. mansoni infected rat sera recognize reduction-sensitive epitopes in recombinant insect cell extracts containing SmLy6 proteins.
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posted on 2017-01-17, 17:30 authored by Greice Krautz-Peterson, Michelle Debatis, Jacqueline M. Tremblay, Sergio C. Oliveira, Akram A. Da’dara, Patrick J. Skelly, Charles B. ShoemakerInsect cells engineered to express SmLy6A, SmLy6B, SmLy6C and SmLy6F were extracted in a mild detergent buffer and resolved by SDS-PAGE under non-reducing or reducing conditions, as indicated. Filters with insect cell extracts containing recombinant SmLy6A, B, C or F were probed by anti-FLAG (A) or rat infection sera (1:2000) (B). Detection employed HRP/anti-FLAG tag or HRP/anti-rat IgG. Numbers indicate the positions of migration of molecular mass markers (kDa).
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anti-tegumental antibody responseSchistosoma mansoni infectionStrong Antibody ResponseMajor Tegumental Membrane Proteins Schistosomiasismansoni infection sera antibodiestegumental surface membrane proteinsschistosome antigensSchistosoma mansoni Infectionschistosome tegumental membrane antigens6B6Fadult schistosomesSchistosoma mansoni worm antigensinsect host cellstegumental membrane proteinsschistosomiasis vaccine development
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