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SOX9 expression in canine embryonic gonads was measured by RNA-seq.

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posted on 2017-10-20, 20:05 authored by Vicki N. Meyers-Wallen, Adam R. Boyko, Charles G. Danko, Jennifer K. Grenier, Jason G. Mezey, Jessica J. Hayward, Laura M. Shannon, Chuan Gao, Afrah Shafquat, Edward J. Rice, Shashikant Pujar, Stefanie Eggers, Thomas Ohnesorg, Andrew H. Sinclair

(A) At d37-39, SOX9 expression was significantly greater in XX DSD gonads at risk (filled triangles) compared to XX age-matched control gonads (open triangles), but <2 fold and primarily due to one sample (C3512). Each open circle represents RNA from one embryonic gonad pair, except for d34, which represents RNA from a gonad pool. Differential expression was statistically tested for XX DSD gonads compared to XX age-matched controls at d37-39 and d42-44 (** p-value ≤ 0.00005). (B) At all ages tested, SOX9 expression in XY littermate (filled inverted triangles) and XY age-matched control gonads (open inverted triangles) was much greater than in XX DSD gonads at risk (filled triangles) and XX age-matched controls (open triangles). Each open circle represents RNA from one embryonic gonad pair, except for d34, which represents RNA from a gonad pool. FPKM is Fragments Per Kilobase of transcript per Million mapped reads.

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