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Pha13 is involved in the expression of PhaPR1 and PhaNPR1, and induced by phytohormone treatment.

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posted on 2018-09-13, 18:15 authored by Li Chang, Ho-Hsiung Chang, Jui-Che Chang, Hsiang-Chia Lu, Tan-Tung Wang, Duen-Wei Hsu, Yuh Tzean, An-Po Cheng, Yi-Shu Chiu, Hsin-Hung Yeh

(A and B) Expression level of Pha13, PhaNPR1, and PhaPR1 were analyzed by qRT-PCR from leaves of P. aphrodite infiltrated with agrobacterium carrying vector (Vector); hairpin RNA (hpRNA) vector to knock down Pha13 (hpPha13; A); and overexpression vector of Pha13 (Pha13-oe; B). The RNA level of vector was set to 1. (C) Transient silencing of PhaNPR1. Expression level of PhaNPR1 and Pha13 were analyzed by qRT-PCR from leaves of P. aphrodite infiltrated with agrobacterium carrying the vector (Vector) or hairpin RNA (hpRNA) vector to knock down PhaNPR1 (hpPhaNPR1). The RNA level of the vector was set to 1. (A to C) Mean ± SD; n = 3 biological replicates; *, P < 0.05, Student’s t-test compared to vector. (D to F) Time-course expression of Pha13 under different plant hormone treatments in P. aphrodite. Expression level of Pha13 was analyzed by qRT-PCR from leaves treated with SA (D), JA (E), and ET (F) at different hours (h) post-treatment. Inoculation buffer treatment was used as a mock control. Results of qRT-PCR were relative to that of mock at individual time course for relative quantification. The RNA level at 0 hour was set to 1 for comparison between different time courses. PhaPR1 and PhaNPR1 were used as SA marker genes. PhaJAZ1 and PhaACO2 were used as JA and ET marker genes, respectively. Data represent mean ± SD; n = 3 technical replicates; *, P < 0.05, Student’s t-test compared to 0 h. One representative experiment is shown from at least three replicates of similar results. PhaUbiquitin 10 was used as an internal control for normalization.

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