<i>Pha13</i> is involved in the expression of <i>PhaPR1</i> and <i>PhaNPR1</i>, and induced by phytohormone treatment.

<p>(A and B) Expression level of <i>Pha13</i>, <i>PhaNPR1</i>, and <i>PhaPR1</i> were analyzed by qRT-PCR from leaves of <i>P</i>. <i>aphrodite</i> infiltrated with agrobacterium carrying vector (Vector); hairpin RNA (hpRNA) vector to knock down <i>Pha13</i> (hpPha13; A); and overexpression vector of <i>Pha13</i> (Pha13-oe; B). The RNA level of vector was set to 1. (C) Transient silencing of <i>PhaNPR1</i>. Expression level of <i>PhaNPR1</i> and <i>Pha13</i> were analyzed by qRT-PCR from leaves of <i>P</i>. <i>aphrodite</i> infiltrated with agrobacterium carrying the vector (Vector) or hairpin RNA (hpRNA) vector to knock down <i>PhaNPR1</i> (hpPhaNPR1). The RNA level of the vector was set to 1. (A to C) Mean ± SD; n = 3 biological replicates; *, <i>P</i> < 0.05, Student’s t-test compared to vector. (D to F) Time-course expression of <i>Pha13</i> under different plant hormone treatments in <i>P</i>. <i>aphrodite</i>. Expression level of <i>Pha13</i> was analyzed by qRT-PCR from leaves treated with SA (D), JA (E), and ET (F) at different hours (h) post-treatment. Inoculation buffer treatment was used as a mock control. Results of qRT-PCR were relative to that of mock at individual time course for relative quantification. The RNA level at 0 hour was set to 1 for comparison between different time courses. <i>PhaPR1</i> and <i>PhaNPR1</i> were used as SA marker genes. <i>PhaJAZ1</i> and <i>PhaACO2</i> were used as JA and ET marker genes, respectively. Data represent mean ± SD; n = 3 technical replicates; *, <i>P</i> < 0.05, Student’s t-test compared to 0 h. One representative experiment is shown from at least three replicates of similar results. <i>PhaUbiquitin 10</i> was used as an internal control for normalization.</p>