<i>Pha13</i> is involved in the expression of <i>PhaNPR1</i>-dependent and -independent marker genes.

<p>(A) Expression level of <i>PhaRdR1</i> and <i>PhaGRX</i> were analyzed by qRT-PCR from leaves of <i>P</i>. <i>aphrodite</i> treated with buffer (Mock) or SA, at different times (h) post-treatment. Results of qRT-PCR were relative to that of mock at individual time course for relative quantification. The RNA level at 0 h was set to 1. Data represent mean ± SD; n = 3 technical replicates; *, <i>P</i> < 0.05, Student’s t-test compared to 0 h. One representative experiment is shown from at least three replicates of similar results. <i>PhaUbiquitin 10</i> was used as an internal control for normalization. (B) Expression level of <i>PhaRdR1</i>, <i>PhaGRX</i>, and CymMV accumulation level were analyzed by semi-quantitative RT-PCR from leaves of <i>P</i>. <i>aphrodite</i> inoculated with buffer (Mock) or infected with CymMV (Cy). <i>PhaUbiquitin 10</i> was used as a loading control, and relative expression levels of the corresponding genes are indicated. (C) Expression level of <i>PhaNPR1</i>, <i>PhaPR1</i>, <i>PhaRdR1</i>, and <i>PhaGRX</i> were analyzed by qRT-PCR from leaves of <i>P</i>. <i>aphrodite</i> infiltrated with agrobacterium carrying the vector (Vector) or hairpin RNA (hpRNA) vector to knock down <i>PhaNPR1</i> (hpPhaNPR1). (D and E) Expression level of <i>PhaRdR1</i>, <i>PhaGRX</i>, and CymMV accumulation level were analyzed by qRT-PCR from leaves of CymMV-infected <i>P</i>. <i>aphrodite</i> infiltrated with agrobacterium carrying vector (Vector); hairpin RNA (hpRNA) vector to knockdown <i>PhaRdR1</i> (hpPhaRdR1, D), or <i>PhaGRX</i> (hpPhaGRX, E). (F and G) Expression level of <i>Pha13</i>, <i>PhaRdR1</i>, and <i>PhaGRX</i> were analyzed by qRT-PCR from leaves of <i>P</i>. <i>aphrodite</i> infiltrated with agrobacterium carrying vector (Vector) or hairpin RNA (hpRNA) vector to knockdown <i>Pha13</i> (hpPha13; F), or to overexpress Pha13 (Pha13-oe; G). C to G, The RNA level of vector was set to 1. Data represent mean ± SD; n = 3 biological replicates; *, <i>P</i> < 0.05, Student’s t-test compared to vector. <i>PhaUbiquitin 10</i> was used as an internal control for normalization.</p>