<i>Pha13</i> is induced by CymMV and involved in virus accumulation.

<p>(A) The CymMV accumulation level and expression level of <i>Pha13</i> were analyzed by qRT-PCR in healthy (Mock) and CymMV-infected (Cy-infected) <i>P</i>. <i>aphrodite</i>. The RNA level in the CymMV was set to 1. Data represent mean ± SD; n = 3 biological replicates; *, <i>P</i> < 0.05, Student’s t-test compared to mock. (B and C) Transient silencing or overexpression of Pha13 in CymMV-infected plants. RNA level of <i>Pha13</i> and CymMV were analyzed by qRT-PCR from leaves of CymMV-infected <i>P</i>. <i>aphrodite</i> infiltrated with agrobacterium carrying vector (Vector); hairpin RNA (hpRNA) vector to knockdown <i>Pha13</i> (hpPha13; B); or overexpression vector of Pha13 (Pha13-oe; C). The RNA level of vector was set to 1. Data represent mean ± SD; n = 3 biological replicates; *, <i>P</i> < 0.05, Student’s t-test compared to vector. D, The protein level of Pha13 in WT and transgenic <i>P</i>. <i>equestris</i> (Pha13#27, #29 and #30) expressing Pha13, Pha13-oe (35S::Flag-Pha13), were analyzed by anti-Pha13 antibody. The anti-Tubulin antibody (Anti-Tub) was used as a loading control. (E) Three wild-type plants and three plants derived from the same transformed protocorm were used for analysis. RNA level of <i>Pha13</i> and CymMV were analyzed by qRT-PCR from leaves of WT or transgenic <i>P</i>. <i>equestris</i> (Pha13#27, #29 and #30) inoculated with CymMV. The RNA level of WT was set to 1. Data represent mean ± SD; n = 3 biological replicates; *, <i>P</i> < 0.05, Student’s t-test compared to WT. <i>PhaUbiquitin 10</i> was used as an internal control for normalization.</p>