Pex19 mutants display shortage in M/LCFAs and survive better when fed with MCFA-rich oils.
(A) GC/MS analysis of FAMEs of wild-typic and Pex19 mutant pupae, concentration in nmol/mg. n = 4. Significance tested with ANOVA. (B) Survival upon addition of different natural oils with different fatty acid compositions. n = 5 in groups of 25 individuals. (C) Survival upon addition of M/LC-TAGs. n = 5 in groups of 25 individuals. -Nip: without nipagin. (D) Survival profile with percentage of pupae, adults, and surviving adults upon rescue diet feeding. n = 10 in groups of 25 individuals. Significance tested using Student t test. Genotypes are w-: w1118, Pex19−/−: w1118; Pex19ΔF7/Pex19ΔF7. Error bars represent SD. *p < 0.05; **p < 0.01; ***p < 0.001. Corresponding raw data can be found in supplemental file S1 Data. FAME, fatty acid methyl ester; GC/MS, gas chromatography/mass spectometry; LCFA, long-chain fatty acid; MCFA, medium-chain fatty acid; TAG, triacylglycerol.