<i>Nox</i> overexpression activates canonical Wnt signaling, but does not promote differentiation.

<p><b>(A)</b> Dual-luciferase assay of TCF activity in F9 cells transfected with <i>EV</i> and treated with DMSO or RA, or transfected with pcDNA3.1-<i>Nox1</i> or pcDNA3.1-<i>Nox4</i>. <b>(B)</b> <i>Dab2</i> expression in F9 cells transfected with <i>EV</i> and treated with DMSO or RA, or transfected with pcDNA3.1-<i>Nox1</i>. <b>(C)</b> <i>Dab2</i> expression in F9 cells transfected with <i>EV</i> and treated with DMSO or RA, or transfected with pcDNA3.1-<i>Nox4</i>. <b>(D)</b> Immunoblot analysis for DAB2, TROMA1, and OCT4 in F9 cells transfected with <i>EV</i> and treated with DMSO or RA, or transfected with pcDNA3.1-<i>Nox1</i>, pcDNA3.1-<i>Nox4</i> or both vectors. β-actin was used as a loading control. A total of 3 independent experiments were analyzed and results are presented as mean ± SEM. Letters denote groups of significance (<i>P</i> < 0.05) tested by a One-Way ANOVA followed by a Tukey’s test.</p>