<i>Nox1</i> and <i>Nox4</i> knockdown attenuates RA-induced differentiation.

<p>RNA was collected from F9 cells transfected with scrambled (<i>scr</i>) si-<i>RNA</i>, si-<i>Nox1</i> and/or si-<i>Nox4</i> siRNA and cultured 4 days with RA treatment. <b>(A)</b> <i>Dab2</i> expression of F9 cells transfected with <i>scr</i>, si<i>-Nox1</i>, or si<i>-Nox4</i> and treated with RA. <b>(B)</b> Immunoblot analysis for DAB2, TROMA1, and OCT4 in F9 cells transfected with <i>scr</i>, si<i>-Nox1</i>, or si<i>-Nox4</i> and treated with RA. <b>(C)</b> Densitometric analysis for DAB2, TROMA1, and <b>(D)</b> OCT4 in F9 cells transfected with <i>scr</i>, si<i>-Nox1</i>, or si<i>-Nox4</i> and treated with RA. β-actin was used as a loading control. A total of 3 independent experiments were analyzed and results are presented as mean ± SEM. Letters and symbols denote groups of significance (<i>P</i> < 0.05) tested by a One-Way ANOVA followed by a Tukey’s test.</p>