Nox1 and Nox4 knockdown attenuates RA-induced differentiation.

RNA was collected from F9 cells transfected with scrambled (scr) si-RNA, si-Nox1 and/or si-Nox4 siRNA and cultured 4 days with RA treatment. (A) Dab2 expression of F9 cells transfected with scr, si-Nox1, or si-Nox4 and treated with RA. (B) Immunoblot analysis for DAB2, TROMA1, and OCT4 in F9 cells transfected with scr, si-Nox1, or si-Nox4 and treated with RA. (C) Densitometric analysis for DAB2, TROMA1, and (D) OCT4 in F9 cells transfected with scr, si-Nox1, or si-Nox4 and treated with RA. β-actin was used as a loading control. A total of 3 independent experiments were analyzed and results are presented as mean ± SEM. Letters and symbols denote groups of significance (P < 0.05) tested by a One-Way ANOVA followed by a Tukey’s test.