In vivo Heparanase (HPSE) expression induced by Ischemia and Reperfusion (I/R) kidney injury.

A) Box plot representing relative gene expression of HPSE evaluated by real-time PCR in renal tissue extract from Wild type (WT) mice. Results were normalized to GAPDH expression. Mean ± S.E (error bars) of two separate experiments performed in triplicate. B) Representative immunofluorescence staining for HPSE in cortical renal tissues of WT mice 72 h after sham operation or I/R kidney injury. I/R, ischemia/reperfusion. C) Quantification of the immunofluorescence staining. **p<0.001vs. WT SHAM.