<i>In vitro</i> antibiofilm activity of <i>Murraya koenigii</i> essential oil extracted using supercritical fluid CO<sub>2</sub> method against <i>Pseudomonas aeruginosa</i> PAO1

2015-10-08T08:37:12Z (GMT) by P. Sankar Ganesh Ravishankar Rai Vittal
<div><p>The antibiofilm activity of <i>Murraya koenigii</i> essential oil (EO) against <i>Pseudomonas aeruginosa</i> PAO1 was investigated in this study. A decrease in the production of rhamnolipid, extracellular polymeric substance and swarming motility was observed by the EO treatment (0.3% v/v). The static microtitre plate assay revealed 80% reduction in biofilm formation by <i>P. aeruginosa</i> PAO1 on <i>M. koenigii</i> EO treatment. Fluorescence microscopy and scanning electron microscopy analyses confirmed the reduction of biofilm formation in <i>P. aeruginosa</i> PAO1 when treated with <i>M. koenigii</i> EO. Gas chromatography–mass spectrometry analysis of the EO revealed the presence of well-known antibiofilm agents such as spathulenol (5.85%), cinnamaldehyde (0.37%) and linalool (0.04%). Cinnamaldehyde has not been previously reported in <i>M. koenigii</i> EO. The potent antibiofilm properties of <i>M. koenigii</i> EO may be effectively exploited in food and pharmaceutical industries as well as in controlling <i>Pseudomonas</i> biofilms on indwelling medical devices.</p></div>