In situ microdissection expression contributing to epithelial-mesenchymal transition (EMT) and collagen production in KD.
A) Schematic diagram of the differentially expressed genes (DEG) in KD that contribute to an activated, hyper-proliferative and inflammatory epidermis. Also depicted are DEG from our microarray data hypothesised to contribute to EMT through upregulation (green arrow) or downregulation (red arrow), which are described along with the upstream regulators identified on enrichment analysis of our microarray data and which have been previously implicated in the EMT process. B) Schematic diagram depicting where ADAMTS and BMP cleave the procollagen peptides to form tropo-collagen and allow collagen fibril assembly necessary for collagen turnover. Once a collagen monomer, it may bind COMP. Collagen production in KD may be increased by the potential existence of positive feedback loops between ADAMTS2/COMP/ADAM12 and TGFβ. ADAM, a disintegrin and metalloproteinase; ADAMTS, a disintegrin and metalloproteinase with thrombospondin motifs; AKR1B10, aldo-keto reductase family 1, member 10; BMP, bone morphogenetic protein; CLDN, claudin; COMP, cartilage oligomeric protein; FGF, fibroblast growth factor; HOX, homeotic gene subset; IL, interleukin; K, keratin; LIMS2, LIM zinc finger domain containing 2; MAPK, mitogen-activated protein kinase; MUCL1, mucin-like 1; S100A8, S100 calcium-binding protein A8; TGFβ, transforming growth factor beta; WDR66, WD repeat domain 66; ZEB, zinc finger E-box-binding proteins.