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In silico analysis of transcriptional activity associated with array peptides with significantly altered phosphorylation levels.

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posted on 2018-01-19, 21:09 authored by Unni Gopinathan, Kathrine Røe Redalen, Anne-Marie Trøseid, Peter Kierulf, Petter Brandtzaeg, Anne Hansen Ree, Jens Petter Berg, Reidun Øvstebø

The figure displays a sub-cellular layout of array peptides with phosphorylation levels significantly altered by lysates from human monocytes stimulated with N. meningitidis. IPA was used to identify transcriptional activity of these peptides (see Material and Methods for detailed algorithm). The kinase dual specificity tyrosine-(Y)-phosphorylation-regulated kinase 1A (DYRK1A) was identified to have transcriptional activity linked with the genes CDC6, CDK1, E2F1, E2F8, MCM3, MCM4, MYBL2, KNTC1, and UBEC2. The overlay function in IPA did not identify any of these genes to be regulated by N. meningitidis (green indicates down-regulation by N. meningitidis).

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