<i>CTPsyn</i> knockdown supresses <i>Myc</i>-induced overgrowth phenotype in <i>Drosophila</i> follicle cells.

<p><b>(A-E)</b> The nuclei of cells both overexpressing <i>Myc</i> and knocking down <i>CTPsyn</i> (<i>UAS-Myc</i>, <i>CTPsyn</i><sup><i>RNAi</i></sup>) are marked by GFP (<b>B</b>, outlined by yellow lines in <b>A-E</b>). <i>Myc</i> overexpression is verified by immunostaining with an antibody against Myc (<b>D</b>). <i>CTPsyn</i> knockdown is verified by immunostaining with an antibody against CTPsyn (<b>C</b>). Note that no cytoophidia are detectable in the clonal cells even when <i>Myc</i> is overexpressed. (<b>F</b>) Quantification of mid-stage follicle cells shows that <i>Myc</i> overexpression (<i>UAS-Myc</i>) alone increases nuclear size significantly. Follicle cells in <i>UAS-Myc</i>, <i>CTPsyn</i><sup><i>RNAi</i></sup> cells have similar nuclear size compared to non-clonal (nc) cells. <i>CTPsyn</i><sup><i>RNAi</i></sup> or <i>CTPsyn</i> overexpression show no significant difference in nuclear area (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005867#pgen.1005867.s010" target="_blank">S10</a> and <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1005867#pgen.1005867.s011" target="_blank">S11</a> Figs for representative images). Quantification represents the mean nuclear areas from > 50 cells in > 3 egg chambers per genotype. ***P<0.001. n.s. = not significant. Error bars show SEM.</p>