<i>B</i>. <i>bronchiseptica</i> LapG cleaves <i>P</i>. <i>fluorescen</i>s LapA and <i>B</i>. <i>bronchiseptica</i> BrtA.

<p>(A) Cleavage of LapA-HA from the surface of <i>P</i>. <i>fluorescens</i> cells with <i>B</i>. <i>bronchiseptica</i> LapG. Western blot with anti-HA antibodies were used to detect LapA-HA of <i>P</i>. <i>fluorescens</i> released into the supernatant fraction for each indicated strain. (B) Cleavage of N-terminal fragment of BrtA by <i>B</i>. <i>bronchiseptica</i> LapG <i>in vitro</i>. Samples correspond to reaction mixture of <i>E</i>. <i>coli</i> lysate expressing indicated protein or empty vector with <i>E</i>. <i>coli</i> lysate expressing the N-terminus of BrtA (N-term BrtA) incubated at room temperature for 1h. Western blot with anti-HA antibodies was used to detect N-term BrtA.</p>