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Aggregatibacter actinomycetemcomitans Growth in Biofilm versus Planktonic State: Differential Expression of Proteins
journal contribution
posted on 2017-07-14, 00:00 authored by Arancha Llama-Palacios, Oksana Potupa, María C. Sánchez, Elena Figuero, David Herrera, Mariano SanzAggregatibacter actinomycetemcomitans (Aa) is
a pathogenic bacterium residing in the subgingival plaque biofilm
strongly associated with the pathogenesis of periodontitis. The aim
of this investigation was to study the protein differential expression
of Aa when growing on biofilm compared with planktonic state using
proteomic analysis by the 2D-DIGE system. Eighty-seven proteins were
differentially expressed during biofilm growth (1.5-fold, p < 0.05), with 13 overexpressed and 37 down-expressed.
Those repressed were mainly proteins involved in metabolism, biosynthesis,
and transport. The overexpressed proteins were outer membrane proteins
(OMPs) and highly immunogenic proteins such as YaeT (OMP), FtsZ, OMP39,
OMP18/16, the chaperone GroEL, OMPA, adenylate kinase (Adk), and dihydrolipoamide
acetyltransferase. The enrichment fractions of the OMPs from biofilm
and planktonic states were obtained, and these proteins were analyzed
by Western blotting with human serum from a periodontitis patient
and one healthy control. These immunogenic proteins overexpressed
in the biofilm may represent candidate virulence factors.
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immunogenic proteinsOMPAimmunogenic proteins overexpressedchaperone GroELplanktonic stateAggregatibacter actinomycetemcomitans Growthproteomic analysismembrane proteinsProteins Aggregatibacter actinomycetemcomitanscandidate virulence factorsperiodontitis patientsubgingival plaque biofilm2 D-DIGE system37 down-expresseddihydrolipoamide acetyltransferaseEighty-seven proteinsOMPDifferential Expression13 overexpressedenrichment fractionsoverexpressed proteinsadenylate kinasePlanktonic Stateplanktonic states
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