ALP1 is widely expressed and its protein product is nuclear-localised.
(A) Complementation assay in clf-50 alp1-4 background. The pALP1::ALP1-GFP transgene fully complements alp1-4 and restores the clf phenotype, whereas pALP1::ALP1-GUS gives weaker complementation so that plants retain a partially suppressed clf phenotype. (B-G) Histochemical staining showing pALP1::ALP1-GUS activity in rosettes (B), leaves (C), roots (D) and inflorescences (E). (F–G) pALP1::ALP1-GFP is nuclear localised in roots (G), whereas a control 35S:GFP construct shows more diffuse localisation in cytoplasm and nucleus (F). Scale bars are 1cm in A, 1mm in B-E and 20μm in F,G.