hUCBSC treatment downregulates cyclin D1 in vitro and in vivo.
(A) Immunocytochemistry of single and co-cultures of U251 and 5310 cells for the expression of cyclin D1 and CD81. Cyclin D1 is conjugated with Alexa Fluor-594 (red), and CD81 is conjugated with Alexa Flour-488 (green). Inset pictures show magnified images. Bar = 200 µm (B) Nude mice with pre-established intracranial human glioma tumors (U251 or 5310) were treated with hUCBSC by intracranial injection (2×105). Fourteen days after hUCBSC administration, the brains were harvested and sectioned and immunoprobed for cyclin D1 and appropriate secondary antibodies. Inset pictures show magnified images. Bar = 200 µm. Each experiment was performed in triplicates with each sample (n = 3).