gAd enhances the motility of mSAT.
2012-05-18T01:40:30Z (GMT) by
<p><b>A</b>) Wound healing assay. Confluent mSAT were serum deprived overnight and then we performed an artificial wound. Cells were treated with free-serum medium (control), free-serum medium containing gAd (1 ug/ml) or 10% FCS. Cells were photographed after 5 hours. Black lines are indicative of the remaining gap in the wound. <b>B</b>) Migration of mSAT induced by gAd. mSAT were serum deprived overnight and then seeded in the upper chamber of the Boyden assay in free-serum medium with or without gAd (1 µg/ml). Four days differentiated C2C12 myotubes were cultivated in the lower chamber and used as chemo-attractant, *p<0,001 <i>vs</i> control. <b>C</b>) The invasiveness assay was performed as described in B, except for the presence of a thin layer of Matrigel in the upper chamber, *p<0,001 and ♦p<0,01 <i>vs</i> control. <b>D</b>) gAd enhances the production of MMP-2. mSAT were treated for 18 h with serum-free medium with or without gAd (1 µg/ml) and the corresponding media were subjected to zymography analysis. *p<0,001 <i>vs</i> control. <b>E</b>) The invasiveness assay of mSAT in response to gAd was performed as in C, except for the presence of the Ilomastat (broad MMP inhibitor) or aprotinin (uPA inhibitor), *p<0,001 and ♦p<0,01 <i>vs</i> control. Representative images of migrated mSAT, after staining with hematoxylin-eosin, are shown in B, C and E. Bar graphs represent the mean of migrated cells counted in six different fields for each experiment.</p>