fat-6 mRNA levels are induced through NHR-80 in glp-1(e2141ts) animals.

(A) fat-5 mRNA levels in wild type, glp-1(e2141ts), glp-1(e2141ts);nhr-80(tm1011), and daf-16(mu86);glp-1(e2141ts) mutants. (B, C, and D) Similar analyses were performed for fat-6, fat-7, and K04A8.5. fat-5, fat-6, and K04A8.5 mRNA levels are up-regulated in glp-1(e2141ts) mutants compared to wild type (9.28-, 5.48-, and 7.25-fold, respectively; Wilcoxon rank-sum test p value <0.05 for all). In contrast, fat-7 mRNA levels are decreased in glp-1(e2141ts) mutants (6.6-fold Wilcoxon rank-sum test p value <0.001). fat-5 mRNA levels are reduced 7.28-fold in daf-16(mu86);glp-1(e2141ts) mutants and 1.7-fold in glp-1(e2141ts);nhr-80(tm1011) mutants compared to glp-1(e2141ts) mutants (Wilcoxon rank-sum test p value <0.05 for both). When compared to wild type levels, fat-5 is induced 1.29-fold in daf-16(mu86);glp-1(e2141ts) mutants and 5.48-fold in glp-1(e2141ts);nhr-80(tm1011) double mutants (Wilcoxon rank-sum test p value <0.05 for both; error bars are standard deviation; *p<0.1, **p<0.05, ***p<0.01, Wilcoxon rank-sum test). In contrast, fat-6 mRNA levels are reduced 1.6-fold in a daf-16(mu86);glp-1(e2141ts) mutant and 4.9-fold in glp-1(e2141ts);nhr-80(tm1011) mutants compared to glp-1(e2141ts) mutants (Wilcoxon rank-sum test p value <0.05 and non-significant, respectively). When compared to wild type levels, fat-6 is induced 2.9-fold in daf-16(mu86);glp-1(e2141ts) double mutants and 0.96-fold in glp-1(e2141ts);nhr-80(tm1011) double mutants (Wilcoxon rank-sum test p value <0.05 and non-significant, respectively; error bars are standard deviation; *p<0.1, **p<0.05, ***p<0.01, Wilcoxon rank-sum test). Relative to glp-1(e2141ts) mutants, K04A8.5 is decreased 6.4-fold in daf-16(mu86);glp-1(e2141ts) mutants (p<0.01) and 1.4-fold in glp-1(e2141ts);nhr-80(tm1011) double mutants (Wilcoxon rank-sum test p value is non-significant). Finally, we found that fat-7 mRNA levels are unchanged in daf-16(mu86);glp-1(e2141ts) and in glp-1(e2141ts);nhr-80(tm1011) mutants relative to glp-1(e2141ts) mutants (Wilcoxon rank-sum test p value is non-significant). Thus, the induction of fat-5 and K04A8.5 is DAF-16 dependant while that of fat-6 depends on NHR-80 only.

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