elt-2 is essential for atf-7-dependent immune gene regulation.

A. Survival curves for wildtype and atf-7(qd22qd130) animals fed with designated RNAi’s during development followed by infection. Averages ± SDs for three plates (N = 129–140 per group) in a representative experiment of several others with similar results. B-E, Gene expression (log scale) in wildtype, pmk-1(km25), and atf-7(qd22qd130) animals, fed with designated RNAi’s during development. Models depict for each panel the mode of disruption, or status, of examined factors (solid-line crosses, loss-of-function mutants; dashed-line crosses, knock-down), placing ELT-2 tentatively at the proximal promoter of immune genes (Gene X) putatively regulated by PMK-1 and ATF-7; atf-7 is depicted as an activator (arrow) or repressor (blunt-ended arrow), based on disruption effects on gene expression. RNA levels were measured in L4/YA worms. Each panel presents averages and SDs for two independent experiments. Asterisks mark inter-group significance with *p<0.05, **p<0.01, and NS, non-significant (paired t-test) for all genes in the group or in underlined subset.