<i>dhfr</i> triple mutant H<sub>e</sub> changes over time (51I/108N/164L).

<p>The figure shows variation in H<sub>e</sub> surrounding <i>dhfr</i> for parasites carrying the 51I/108N/164L mutant during the 1980s (n = 8) and 1990s (n = 19). H<sub>e</sub> surrounding this allele appears to have increased between the two periods. The two flat, dashed lines represent two different estimates of neutral H<sub>e</sub> in Brazil.</p>