Work-flow for construction of the primary self-replicating wide-host-range vector pDF-trc.

<p>The unique restriction sites (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050470#pone-0050470-t001" target="_blank">Table 1</a>) for exchange of different genetic elements (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050470#pone-0050470-t002" target="_blank">Table 2</a>) are shown in red.</p>