Whole-cell recordings show that CA1 pyramidal neurons and striatal neurons undergo terminal AD induced by OGD or ouabain exposure.

A1) In response to 10 minutes of OGD the CA1 neuron initially hyperpolarizes before rapid AD (arrow) to near-zero millivolts from which it does not recover. Inset shows typical voltage responses of a CA1 neuron in response to hyperpolarizing and depolarizing current pulses. A2) CA1 neuron exposed to 10 minutes of 100 µM ouabain. The neuron initially depolarizes and discharges, leading to spike inactivation just prior to a rapid AD-like response (arrow) from which the neuron does not recover. B1) Tonic firing by three striatal neurons in response to a depolarizing current pulse. B2) In response to OGD a striatal neuron slowly depolarizes before undergoing rapid AD to near-zero millivolts. Upon return to control aCSF, the neuron begins to repolarize, but does not recover. B3) In response to 10 minutes of ouabain exposure, a striatal neuron slowly depolarizes before a rapid AD-like response to near-zero millivolts (arrow). Upon return to control aCSF there is no recovery.