Figure_3.tif (112.71 kB)
Western blotting using α-PA antibody.
figure
posted on 2008-11-21, 01:45 authored by Adam C. Wilson, Magali Soyer, James A. Hoch, Marta PeregoStrains grown in R Media with 0.8% NaHCO3 under 5% CO2 at 37°C supplemented with Erythromycin and Lincomycin as appropriate. Amount of sample loaded on 10% SDS-PAGE gel was normalized relative to cell density. Lane 1, MagicMark XP; Lane 2, 34F2 strain; Lane 3, 34F2ΔBAS2714-12 strain; Lane 4, 34F2ΔBAS2714-12 strain carrying plasmid pHT315; Lane 5, 34F2ΔBAS2714-12 strain carrying plasmid pAW144.
