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Western blots comparing losartan and water groups.

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posted on 2015-10-27, 03:50 authored by Harry A. Quigley, Ian F. Pitha, Derek S. Welsbie, Cathy Nguyen, Matthew R. Steinhart, Thao D. Nguyen, Mary Ellen Pease, Ericka N. Oglesby, Cynthia A. Berlinicke, Katherine L. Mitchell, Jessica Kim, Joan J. Jefferys, Elizabeth C. Kimball

Immunoblots for pERK1/2, ERK1/2, pSmad2, pSmad3, Smad2/3 and GAPDH for 12 scleral specimen groups. (1) water control; (2) losartan control; (3) fellow eyes of water-treated glaucoma; (4) fellow eyes of losartan-treated glaucoma; (5) water-treated glaucoma; and (6) losartan-treated glaucoma. Graphs show quantification by densitometry of blots, indicating the ratio of the activated molecules to the total amount of that molecular species: pERK/total ERK, pSmad2/total Smad2/3, and pSmad3/total Smad2/3. The comparisons are among all types of glaucoma eyes compared to naïve control eyes at 3 days (left group) and 1 week (right group) after glaucoma. Water-glaucoma group in blue shows 3-fold elevation in pERK/ERK ratio, while no difference from control is seen in losartan-glaucoma eyes. At one week, both water and losartan glaucoma eyes have substantial reduction in pERK/ERK ratio compared to controls. Very mild increases are seen in pSmad ratios in either water or losartan groups. See S1 Fig for original western blots.

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