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Western blot profile of ME7 brain homogenate digested with EF with and without BS.

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posted on 2013-07-16, 02:42 authored by Emeka A. Okoroma, Diane Purchase, Hemda Garelick, Roger Morris, Michael H. Neale, Otto Windl, Oduola O. Abiola

Lanes 2, 3, 4, and 5 are ME7 scrapie brain homogenate digested at 65°C for 1 h with PK (100 µg/ml), EF+BS, BS and EF respectively. Total removal of PrPSc signal was achieved with EF+BS only. PrPSc was probed with SAF83 mAb.

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