Western blot of bacterial lysates probed with the corresponding sera.

A: bacterial lysates probed with the serum of FMF190(A); B – with C110; C – with FMF87(R); and D – with C111. In A and B: line 1 – B. ovatus, line 2 – P. distasonis, line 3 – Bacteroides sp., line 4 – L. reuteri, line 5 – E. coli, and line M – biotinylated molecular weight standard mixture SDS-6B (Sigma-Aldrich, UK). In C and D: line 1 – E. hirae, line 2 – L. reuteri, line 3 – B. ovatus, and line M – biotinylated molecular weight standard mixture SDS-6B (Sigma-Aldrich, UK).