Western blot analysis of phosphate-induced MAPK in S2R+ and Kc167 cells.

<p><b>A:</b> Dose response and time course of phosphate-activated MAPK in S2R+ cells. <b>B:</b> Dose response of phosphate-activated MAPK in S2R+ cells. <b>C:</b> Time course of phosphate-activated MAPK in S2R+ cells. <b>D:</b> Time course of phosphate-activated MAPK in Kc167 cells. <b>E:</b> Effect of PFA on activation of MAPK in S2R+ cells Shown are one representative Western blot autoradiogram (A), or pooled densitometic data of at least three independent Western blot experiments (B–E). Abbreviations: Pi = inorganic phosphate, Ins = human insulin, Ly = Ly294002 (PI3K-inhibitor, 50 uM), PFA = phosphonoformic acid (30 mM, unless otherwise noted), S10 = sodium sulfate (10 mM), P10 = sodium phosphate (10 mM).</p>