Western blot analysis of Morgue-associated proteins confirms Morgue association with polyubiquitin and SkpA.

<p><b>A.</b> Silver stained SDS gel of proteins from P[<i>da</i>-Gal4], P[UAS-Morgue] or P[<i>da</i>-Gal4],P[UAS-Morgue-3xFLAG] adult flies. Note that apparent molecular weight of Morgue-3xFLAG (arrowhead) is greater than native Morgue. Note smaller proteins (lower arrowheads) that are present in Morgue-3xFLAG expressing flies. <b>B.</b> Anti-SkpA Western blot of proteins purified from P[<i>da</i>-Gal4], P[UAS-Morgue] or P[<i>da</i>-Gal4], P[UAS-Morgue:3xFlag] flies via anti-FLAG chromatography. Note prominent SkpA band (arrowhead) present in proteins purified from P[<i>da-</i>Gal4], P[UAS-Morgue:3xFlag]. A shared background band of approximately 30 kDa is present in both samples. <b>C.</b> Anti-ubiquitin Western blot of proteins purified from P[<i>da</i>-Gal4], P[UAS-Morgue] or P[<i>da</i>-Gal4], P[UAS-Morgue:3xFlag] flies via anti-FLAG chromatography. Note ubiquitin band (arrowhead) present in proteins purified from P[<i>da</i>-Gal4], P[UAS-Morgue:3xFlag].</p>