Figure_1.tif (2.8 MB)
Western blot analysis and dot blot analysis.
figure
posted on 2013-02-20, 12:34 authored by Paola Neri, Shunji Tokoro, Ryo Kobayashi, Tsuyoshi Sugiyama, Kouji Umeda, Takeshi Shimizu, Takao Tsuji, Yoshikatsu Kodama, Keiji Oguma, Hiroshi Mori(A–C) Western blot analysis of anti-Stx-1 IgY and anti-Stx-2 IgY. hpStx-1 and hpStx-2 were subjected to SDS-PAGE at 2.5 µg/lane. (A) CBB staining of SDS-PAGE gel. (B) Anti-Stx-1 IgY and (C) anti-Stx-2 IgY were applied to the membrane at 20 µg/ml. The marks in the figures indicate A subunit, A1 fragment and monomeric B subunit. (D, E) Dot blot analysis of anti-Stx-1 IgY and anti-Stx-2 IgY. hpStx-1 and hpStx-2 were blotted at 4 µg/dot, and Stx-1BH and Stx-2BH at 2 µg/dot. Anti-Stx-1 IgY and anti-Stx-2 IgY were applied to the membrane at 2 µg/ml and 4 µg/ml, respectively.
