Figure_4.tif (614.67 kB)
WG-DASL assay performance with low RNA inputs.
figure
posted on 2009-12-03, 00:57 authored by Craig April, Brandy Klotzle, Thomas Royce, Eliza Wickham-Garcia, Tanya Boyaniwsky, John Izzo, Donald Cox, Wendell Jones, Renee Rubio, Kristina Holton, Ursula Matulonis, John Quackenbush, Jian-Bing Fan(A) Raw intensity scatterplots are shown for assay replicates for 250 pg Raji RNA and (B) also for a correlation between 250 pg and 100 ng Raji RNA. (C) Lists of differentially expressed probes were generated by comparing replicate data for Raji and MCF-7 cells using a cutoff of p = 0.001. The overlap of differentially expressed probes (Raji vs. MCF-7) between the 250 pg and 100 ng RNA inputs was calculated as the percentage of differentially expressed probes identified in the 250 pg sample which was also identified in the 100 ng sample.
