Virions harvested from siRNA treated cells are efficiently depleted of the siRNA-targeted protein.

143B cells were transfected with Lipofectamine only or siRNAs against the control VP16 or the positive hits Arf1, DDX3X and eIF4H. They were then infected at a MOI of 5 with HSV-1 K26GFP and the supernatants collected at 24 hours. Concentrated supernatants corresponding to equal amounts of VP5 were analyzed and 20 µg of Lipofectamine-treated cell lysates were used as control. The reduced levels of A) VP16, B) ARF1, C) DDX3X and D) eIF4H in newly produced virions in the supernatants were confirmed by Western blotting.