Variations in the organization of nuclear content between normal (left), fibrocystic (middle) and metastatic (right) mammary epithelial cells (scale bar = 5 microns).

Light gray haze depicts cytoplasm. Nuclear membrane is blue, low density regions of chromatin are green, moderate density regions are yellow, high density regions are orange, and nucleoli are magenta. Increased clumpiness is apparent in abnormal cells, and metastatic cell contains large number of smaller high-density clumps. Opacity of nuclear membrane (in blue) has been deliberately decreased to enable viewing of nuclear interior. Corresponding videos of these renderings are available in Movies S3, S4, S5.