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Table_2.xls (9.5 kB)

Values of parameters used in simulations.

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posted on 2013-02-25, 00:20 authored by Juan M. Fernández, Gisela Di Giusto, Maia Kalstein, Luciana Melamud, Valeria Rivarola, Paula Ford, Claudia Capurro

Data were obtained either from our own measurements in MIO-M1 cells or from literature, as follows: i- Vcell and Ac in isotonic conditions were estimated from confocal images as previously reported [43]; ii- Pf was obtained from the measured cell volume changes during a hypoosmotic challenge using a modification of the Fick’s law [20]; iii- Vm corresponds to the value recorded using sharp electrode patch clamp technique by Limb et al [19]; iv- intracellular Na+, K+ and Cl concentration are the typical values for most cell types; v- extracellular Na+, K+ and Cl concentrations are the same as in experimental solutions; vi- ion permeabilities were chosen in order to obtain the Vm value of MIO-M1 cells; vii- the initial parameters presented for the Mannitol condition were obtained by simulating an extracellular solution change, from ISONaCl to ISOMannitol (see Materials and Methods section).

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