Validation of dysregulated miRNAs in sporadic versus familial papillary thyroid cancer by quantitative RT-PCR.

MiR-20a was 4-fold and miR-886-3p was 3-fold higher in familial papillary thyroid cancer as compared to sporadic (p = 0.025 for miR-20a, p = 0.028 for miR-886-3p, p = 0.37 for miR-195, p = 0.46 for miR-29c). Values are average expression plus and minus the standard error of mean. The Y axis represents the ratio of miRNA and U6 RNA using 2^−ΔΔCt method, and the lowest value of the sample was set to 1.0.