Validation of candidate genes from ChIP-seq via QT-PCR in STAT5A and STAT5B knock-down human CD4⁺ T cells.

The expression levels were compared STAT5A KD or STAT5B KD CD4⁺ T cells with control CD4⁺ T cells. Genes regulated similarly by both STAT5A and STAT5B are SGK1, GTF2H5 and SLC22A5. Genes regulated specifically by STAT5A are NDRG1, DNAJC6, ST3GAL1, SAMD4A, SSH2, MAP3K5 and BCL2L1. Genes regulated specifically by STAT5B are DOCK8, SNX9, SKAP1, TNFSF10, FOXP3, IL2RA and UGCG. Data is presented as mean ± SEM. *, P<0.01; **, P<0.001. There were no statistical differences if not annotated. Abbreviations: SGK1, serum/glucocorticoid regulated kinase 1; GTF2H5, general transcription factor IIH, polypeptide 5; BCL2L1, BCL2-like 1; SLC22A5, solute carrier family 22 (organic cation/carnitine transporter), member 5; CDKAL1, CDK5 regulatory subunit associated protein 1-like 1; DNM2, dynamin 2; DUSP5, dual specificity phosphatase 5; MBP, myelin basic protein; ARL4C, ADP-ribosylation factor-like 4C; NDRG1, N-myc downstream regulated 1; DNAJC6, DnaJ (Hsp40) homolog, subfamily C, member 6; ST3GAL1, ST3 beta-galactoside alpha-2,3-sialyltransferase 1; SAMD4A, sterile alpha motif domain containing 4A; SSH2, slingshot protein phosphatase 2; MAP3K5, mitogen-activated protein kinase kinase kinase 5; CBS, cystathionine-beta-synthase; PPP2R2B, protein phosphatase 2, regulatory subunit B, beta; DOCK8, dedicator of cytokinesis 8; SNX9, sorting nexin 9; SKAP1, src kinase associated phosphoprotein 1; PTGER1, prostaglandin E receptor 1 (subtype EP1), 42 kDa; DIDO1, death inducer-obliterator 1; TNFSF10, tumor necrosis factor (ligand) superfamily, member 10; FOXP3, forkhead box P3; IL2RA, interleukin 2 receptor, alpha; UGCG, UDP-glucose ceramide glucosyltransferase; LNPEP, leucyl/cystinyl aminopeptidase; PPP1R3F, protein phosphatase 1, regulatory subunit 3F.