Urothelium-like phenotype differentiation of ASCs.

<p>Immunofluorescent staining detection of UP-IA and UP-II in differentiated ASCs. The differentiation efficiency of induction by conditioned medium and transwell system for 7 and 21 days. The quantification of mRNA for the urothelium-specific marker UP-IA in ASCs after induction by conditioned media for 7 and 21 days. A, G: UP-IA and UP-II expression in urothelium. B, H: ASCs did not express UP-IA or UP-II. C, I, E, K: ASCs expressed UP-IA and UP-II in a small amount after induction by CM or transwell system for 7 days. D, J, F, L: UP-IA and UP-II expression in ASCs after induction for 21 days by conditioned medium or transwell system. M: The difference in the ASC differentiation efficiency between the two different induction methods at the 7<sup>th</sup> and 21<sup>st</sup> day. N: The quantification of UP-IA mRNA in human ASCs after induction by conditioned media for 7 and 21 days. CM = conditioned medium, TW = transwell indirect co-culture, Scale bar = 20 µm.</p>