Figure_9.tif (1.83 MB)

Up-regulation of Stmn1 in human breast cancer tissues.

figure

posted on 2012-10-11, 00:26 authored by Rui Wang, Hong-Bin Wang, Chan Juan Hao, Yi Cui, Xiao-Chen Han, Yi Hu, Fei-Feng Li, Hong-Fei Xia, Xu Ma

We pooled two patients' cancer tissues as a sample. (A) The protein level of STMN1 in human breast cancer tissues was detected by western blot. The bands were analyzed using the Quantity One analyzing system (Bio-Rad Laboratory inc.). The expression of β-ACTIN served as an internal control. The black histograms represent the expression level of STMN1 in breast cancer tissues of all (A1), ER/PR−,HER2−(A2), ER/PR+, HER2+ (A3), ER/PR+, HER2− (A4) and ER/PR−,HER2+(A5) and expressed as STMN1 intensity/β-ACTIN intensity to normalize for gel loading and transfer. N represents non-tumor tissues, and T represents tumor tissues. (B) The mRNA level of Stmn1 in human breast cancer tissues and adjacent normal breast tissues was detected by qRT-PCR. The expression level of Stmn1 was obviously increased in 83.33% individual sample. Statistical analyses were performed to analyze the overall trend of Stmn1 in human breast cancer tissues of all (B1), ER/PR−,HER2− (B2), ER/PR+, HER2+ (B3), ER/PR+, HER2− (B4) and ER/PR−, HER2+ (B5). β-actin serves as an internal reference. The y-axis displays the relative log2 ratio of Stmn1 normalized to β-actin. *P<0.05, **P<0.01.