Translational inhibition of APP and tau is mediated via the 5′ UTR.

Firefly luciferase reporter plasmids containing the 5′ UTR of APP, tau, SOD1 or TXN upstream of a destabilized firefly luciferase open reading frame were transfected into SH-SY5Y cells +/− 10 µM hippuristanol. The dramatic reduction of luciferase expression in the APP and tau 5′ UTR constructs following hippuristanol treatment is not mirrored by the SOD1 and TXN 5′ UTR containing plasmids. Luciferase activity was normalized to a cotransfected Renilla control reporter and means and standard errors of at least three replicates are shown.