Figure_4.tif (3.75 MB)
Transfection of HEK293FT cells with pEGFP with varying ratios of PLGA.
figure
posted on 2013-09-30, 01:31 authored by Eun Jin Seo, Il Ho Jang, Eun Kyoung Do, Hyo Cheon Cheon, Soon Chul Heo, Yang Woo Kwon, Geun Ok Jeong, Ba Reun Kim, Jae Ho Kim(A) Fluorescence and bright field images (B) flow cytometry analysis of cells at 48 h after transfection with varying ratios of PLGA/bPEI-DNA (PLGA:bPEI:DNA, w/w/w) nanoparticles are shown. (C) Results of gel electrophoresis analysis of bPEI-DNA (bPEI:DNA, w/w, upper panel) and PLGA/bPEI-DNA (PLGA:bPEI:DNA, w/w/w, lower panel) nanoparticles are shown. 1 µg of DNA was applied to each well (transfection, six-well plate) or lane. Scale bar represents 100 µm (*, p < 0.05 by Student’s t-test). n=3.
