Transcript validation of differentially expressed proteins at different development periods.

<p>mRNA of different development days are measured by quantitative real time PCR. Samples are normalized with reference gene (GAPDH) and with the expression abundant on day 13 as the reference sample. Error bar is standard deviation. Gene symbols indicating different genes refer to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020428#pone.0020428.s002" target="_blank">Table S2</a>. Different letters (a, b, c) are significantly different (p<0.05).</p>