TorS and EvgS show distinct associations and clustering properties.

The fluorescence anisotropy (r) measured from MG1655 cells expressing mYFP tagged TorS or EvgS sensors at various expression levels, using 0-100 µM IPTG, which was assumed to be correlated with the total fluorescence. A total fluorescence intensity of 10⁵ counts/second corresponds to approximately 4,000 copies of mYFP per cell, estimated as described in Materials and Methods. Line is a guide to the eye. Typical fluorescence images of these cells are shown on the right.