Time-course profile of ME7 brain homogenate digested with EF+BS at 65°C.

<p>Lane 1 is neat ME7 brain homogenate (positive control) and lane 2 is proteinase K digested sample (77 µg/ml final PK conc.). Lanes 3–6 are digested with EF+BS at 10, 30, 45 and 60 min. Samples were digested at 65°C and PrP<sup>Sc</sup> was probed with SAF83 mAb.</p>