The time course of the effect of βAR stimulation in vivo on phophorylation of Akt, ERK1/2, P70S6K, S6, GSK-3α/β and FOXO3a.

Adult male C57BL/6 mice were treated with vehicle control or isoproterenol (ISO, 1.25 mg/kg, i.p.) for the indicated time. Shown are representative Western blots performed on LV tissue lysates with antibodies against phospho-Akt (Thr308), phospho-Akt (Ser473), phospho-ERK1/2 (Thr202/Tyr204), phospho-P70S6K (Thr389), phospho-P70S6K (Thr421/Ser424), phospho-S6 (Ser235/236), phospho-S6 (Ser240/244), phospho-GSK-3α (Ser21), phospho-GSK-3β (Ser9), and phospho-FOXO3a (Ser318/321). Blots of individual total protein and actin were also included.