The sarcomeric localization of Smyd1b_tv1 occurs after the sarcomere formation in myofibers of zebrafish embryos.

A–C. Immunostaining using sarcomeric specific antibodies against MyHC (A), α-actin (B), and α-actinin (C) in the trunk muscles of zebrafish embryos at 24 hpf. D. Immunostaining using anti-myc antibody shows the primary cytoplasmic localization of Smyd1b_tv1 in the trunk muscles of smyd1b_tv1myc transgenic fish embryos at 24 hpf. Muscle pioneer cells with the sarcomeric localization are indicated by arrows. Scale bar: 30 µm.