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The relative gene expression and subsequent anthocyanin amounts produced during the ripening process.

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posted on 2014-02-05, 04:23 authored by Tae Kyung Hyun, Sarah Lee, Yeonggil Rim, Ritesh Kumar, Xiao Han, Sang Yeol Lee, Choong Hwan Lee, Jae-Yean Kim

(A) Biosynthetic pathway of anthocyanins. Enzyme names were abbreviated as follows: chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), flavonoid 3′-hydroxilase (F3′H), flavonoid 3′5′-hydroxylase (F3′5′H), dihydroflavonol 4-reductase (DFR), leucoanthocyanidin dioxygenase (LDOX) and anthocyanidin 3-O-glucosyltransferase (3GT). (B) Expression pattern of genes involved in anthocyanin biosynthesis pathway. Expression levels of genes from ripening stage 2 and 3 were compared to stage 1. The means and standard errors were calculated from three independent measurements. Student's t test compared with Stage 1, * P<0.05, ** P<0.01 and *** P<0.001. (C) Anthocyanin contents determined by UPLC-Q-TOF-MS were represented by box-whisker plots. Metabolites were relatively quantified by their m/z peak areas with the instrument and data processing. The error bars are the standard deviations from three independent measurements.

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