The purified CMG2-Fc mutants were analyzed by 10% SDS-PAGE under reducing conditions (A, B) or non-reducing conditions (C).

<p>Gels were visualized by Coomassie blue staining (A, C) or subjected to western blotting with a mouse anti-CMG2 antibody (B). Lanes 1, 4 and 6: CMG2-Fc (Y158Q); lanes 2, 5 and 7: CMG2-Fc (E117Q); lanes 3 and 8: protein marker.</p>