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The purified CMG2-Fc mutants were analyzed by 10% SDS-PAGE under reducing conditions (A, B) or non-reducing conditions (C).

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posted on 2014-08-07, 03:06 authored by Yongyi Xi, Xiaojie Wu, Lihua Gao, Yong Shao, Hui Peng, Hongxing Chen, Huipeng Chen, Xianwen Hu, Junjie Yue

Gels were visualized by Coomassie blue staining (A, C) or subjected to western blotting with a mouse anti-CMG2 antibody (B). Lanes 1, 4 and 6: CMG2-Fc (Y158Q); lanes 2, 5 and 7: CMG2-Fc (E117Q); lanes 3 and 8: protein marker.

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